Double Staining for Peeling the ERM and ILM

The best way to peel off not only the ERM, but also the ILM is by getting the best view possible. This is accomplished by staining the ERM with Trypan Blue and the ILM with Brillant Blue G. However, in some cases it is not simple to stain the tissue, that is why we double stain from the beginning to distinguish the ERM and the ILM, otherwise it can cause retinal tears. Moreover, if we cannot see the tissues properly we can cause retinal detachment.